For a proper usage you should place the tube in the anti-burn holder and pour the warm oil sample (at least 60 ºC) up to the line on the label present in the test tube. Shake the tube well (from 30 to 60 seconds) in order to produce a uniform mixture (the color changes when the oil is mixed with the reagent). Allow the mixture to settle for 2 minutes. Hold the tube vertically near a source of light and compare the obtained color to the color scale.

Please note that if the result is on level 5, the oil should be discarded, as it has passed over the legal/recommended limit for polar compounds.

You can watch this procedure on a short video (about 1 minute):

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